Inhibition of mouse acrosome reaction and sperm-zona pellucida binding by anti-human sperm membrane protein 1 antibody.
- Author:
Guo-Yan CHENG
1
;
Jian-Li SHI
;
Min WANG
;
Yan-Qin HU
;
Chun-Meng LIU
;
Yi-Fei WANG
;
Chen XU
Author Information
- Publication Type:Journal Article
- MeSH: Acrosome Reaction; drug effects; immunology; physiology; Animals; Antibodies; pharmacology; Antigens, Surface; genetics; Cloning, Molecular; DNA Primers; Escherichia coli; genetics; Female; Fertilization; Fluorescent Antibody Technique, Indirect; Humans; Male; Membrane Proteins; genetics; Mice; Mice, Inbred BALB C; Recombinant Proteins; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Zona Pellucida; immunology; physiology
- From: Asian Journal of Andrology 2007;9(1):23-29
- CountryChina
- Language:English
-
Abstract:
AIMTo investigate the possible functions of human sperm membrane protein (hSMP-1) in the process of fertilization.
METHODSA 576-bp cDNA fragment of HSD-1 gene coding for the extracellular domain of hSMP-1 was cloned and expressed. The localization of this protein on human and mouse sperm was determined by indirect immunofluorescent staining by using anti-recombinant hSMP-1 (anti-rhSMP-1) antibodies. Sperm acrosome reaction and sperm-zona pellucida (ZP) binding assay were carried out in 10-week-old BALB/c mice.
RESULTSRecombinant hSMP-1 was successfully cloned and expressed. The expression of the native protein was limited on the acrosome of human and mouse sperm. Treatment of anti-rhSMP-1 antibodies significantly decreased the average number of sperms bound to each egg. Meanwhile, the percentage of acrosome reaction was decreased in comparison to pre-immune control after treatment with anti-rhSMP-1 (P < 0.05).
CONCLUSIONThe results suggest that anti-rhSMP-1 antibody inhibited mouse acrosome reaction and sperm-ZP binding.
