Expression and localization of CKLFSF2 in human spermatogenesis.
- Author:
Gang LIU
1
;
Zhong-Cheng XIN
;
Liang CHEN
;
Long TIAN
;
Yi-Ming YUAN
;
Wei-Dong SONG
;
Xue-Jun JIANG
;
Ying-Lu GUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibody Specificity; COS Cells; Cercopithecus aethiops; Chemokines; biosynthesis; immunology; Endoplasmic Reticulum; metabolism; Germ Cells; metabolism; Humans; Immunohistochemistry; In Situ Hybridization; Infertility, Male; metabolism; MARVEL Domain-Containing Proteins; Male; Meiosis; Microscopy, Confocal; Spermatogenesis; physiology; Testis; metabolism
- From: Asian Journal of Andrology 2007;9(2):189-198
- CountryChina
- Language:English
-
Abstract:
AIMTo investigate the expression and subcellular localization of chemokine-like factor superfamily 2 (CKLFSF2) in human testis and its potential role in spermatogenesis.
METHODSA specific polyclonal antibody against CKLFSF2 was raised. The expression and cellular localization of CKLFSF2 in the seminiferous tubules was checked by immunohistochemistry method. Also, in situ hybridization was applied to localize the mRNA distribution. The EGFP-CKLFSF2 fusion protein was expressed in COS-7 cells to localize its subcellular location in vitro. In addition, the abnormal expression of CKLFSF2 in testes of patients with male infertility was assayed by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry methods.
RESULTSHaving a close correlation with spermatogenesis defects, CKLFSF2 was specifically expressed in meiotic and post-meiotic germ cells, which were localized to the endoplasmic reticulum (ER) near the Golgi apparatus.
CONCLUSIONCKLFSF2 could play important roles in the process of meiosis and spermiogenesis, and might be involved in the vesicular transport or membrane apposition events in the endoplasmic reticulum.
