Gene expression changes of urokinase plasminogen activator and urokinase receptor in rat testes at postnatal stages.
- Author:
Dong-Hui HUANG
1
;
Hu ZHAO
;
Yong-Hong TIAN
;
Hong-Gang LI
;
Xiao-Fang DING
;
Cheng-Liang XIONG
Author Information
- Publication Type:Journal Article
- MeSH: Aging; genetics; Animals; Animals, Newborn; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Male; Polymerase Chain Reaction; Rats; Receptors, Cell Surface; genetics; Receptors, Urokinase Plasminogen Activator; Spermatogenesis; Spermatozoa; enzymology; physiology; Testis; growth & development; physiology; Urokinase-Type Plasminogen Activator; genetics
- From: Asian Journal of Andrology 2007;9(5):679-683
- CountryChina
- Language:English
-
Abstract:
AIMTo investigate the gene expression changes of urokinase plasminogen activator (uPA)/urokinase receptor (uPAR) in rat testes at postnatal stages and explore the effects of uPA/uPAR system on the rat spermatogenesis.
METHODSThe mRNAs of uPA and uPAR in rat testes were measured by using real-time quantitative polymerase chain reaction (PCR) at postnatal days 0, 5, 10, 15, 21, 28, 35, 42, 49 and 56, respectively.
RESULTSThe tendencies of uPA and uPAR mRNA expression were similar at most postnatal stages except for D(0). The expression of uPAR mRNA in rats testes was relatively higher than that of uPA at postnatal D(0), and both were decreased until D(21), increased obviously at postnatal D(28), reached a peak at postnatal D(35), then declined sharply at postnatal D(42) and retained at a low level afterwards.
CONCLUSIONThe uPA/uPAR system may be strongly linked to spermiation and spermatogenesis via regulating germ cell migration and proliferation, as well as promoting the spermiation and detached residual bodies from the mature spermatids.
