Higher frequency of Yq microdeletions in sperm DNA as compared to DNA isolated from blood.
- Author:
Rima DADA
1
;
Rakesh KUMAR
;
M B SHAMSI
;
Rajeev KUMAR
;
Kiran KUCHERIA
;
Raj K SHARMA
;
Satish K GUPTA
;
Narmada P GUPTA
Author Information
- Publication Type:Journal Article
- MeSH: Chromosomes, Human, Y; genetics; DNA; blood; genetics; isolation & purification; Humans; Male; Repetitive Sequences, Nucleic Acid; Sequence Deletion; Spermatozoa; physiology
- From: Asian Journal of Andrology 2007;9(5):720-722
- CountryChina
- Language:English
-
Abstract:
AIMTo determine if Yq microdeletion frequency and loci of deletion are similar in two tissues (blood and sperm) of different embryological origin.
METHODSThe present study included 52 infertile oligozoospermic cases. In each case, DNA was isolated from blood and sperms and polymerase chain reaction (PCR) microdeletion analysis was done from genomic DNA isolated from both the tissues. The PCR products were analyzed on a 1.8% agarose gel. PCR amplifications found to be negative were repeated at least three times to confirm the deletion of a given marker.
RESULTSOnly 1 case harbored microdeletion in blood DNA, whereas 4 cases harbored microdeletion in sperm DNA.
CONCLUSIONThe frequency of Yq microdeletions is higher in germ cells as compared to blood. As the majority of infertile couples opt for assisted reproduction procreation techniques (ART), Yq microdeletion screening from germ cells is important to understand the genetic basis of infertility, to provide comprehensive counseling and most adapted therapeutics to the infertile couple.
