Cortical neuron apoptosis induced by beta-amyloid peptide and protective effect of panoxadiol in mice.
- Author:
Jing ZHOU
1
;
Bai-qiang SUN
;
Rui-lan GAO
;
Li-hua ZHENG
;
Shu-cai LING
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid beta-Peptides; toxicity; Animals; Apoptosis; drug effects; Cells, Cultured; Cerebral Cortex; cytology; Drugs, Chinese Herbal; isolation & purification; pharmacology; Female; Fetus; Ginsenosides; isolation & purification; pharmacology; Mice; Mice, Inbred ICR; Neurons; cytology; Neuroprotective Agents; isolation & purification; pharmacology; Peptide Fragments; toxicity; Pregnancy; Proto-Oncogene Proteins c-bcl-2; metabolism
- From: Journal of Zhejiang University. Medical sciences 2008;37(5):451-455
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the apoptosis of cortical neurons induced by beta-amyloid peptide (Abeta(1-40)) and the protective effect of panoxadiol.
METHODSThe Abeta(1-40) induced damage of primarily cultured mouse cortical neurons was examined with morphological observation, MTT assay, DNA agarose gel electrophoresis and Western-blot.
RESULTAfter 48 h treated with 12 mumol/L Abeta(1-40), the cortical neurons showed apoptotic characteristics: including decreased OD570 value in MTT assay, DNA cleavage fragment in electrophoresis and increased apoptotic cells. Western-blot showed that the expression of bcl-2 reduced significantly (P<0.05). Cell apoptosis was significantly attenuated in 40 mg/L panoxadiol treated group.
CONCLUSIONPanoxadiol can protect cultured cortical neurons from apoptosis induced by Abeta(1-40) in mice.
