5-Lipoxygenase/cysteinyl leukotriene pathway is not involved in injury of rat C6 glioma cells induced by oxygen-glucose deprivation.
- Author:
Xue-qin HUANG
1
;
Xiao-jia HUANG
;
Li-hui ZHANG
;
Ling-ling CHEN
;
Yun-bi LU
;
Wei-ping ZHANG
;
Er-qing WEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arachidonate 5-Lipoxygenase; metabolism; Cell Hypoxia; physiology; Glioma; pathology; Glucose; metabolism; Rats; Receptors, Leukotriene; metabolism; Signal Transduction; physiology; Tumor Cells, Cultured
- From: Journal of Zhejiang University. Medical sciences 2008;37(5):456-462
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine whether oxygen-glucose deprivation (OGD) induces C6 cell injury, and whether 5-lipoxygenase (5-LOX)/cysteinyl leukotriene (CysLT) pathway is involved in OGD-induced injury.
METHODSAfter OGD treatment and recovery for various durations, the viability of C6 cells was determined, and the effects of 5-LOX inhibitors and CysLT receptor antagonists were investigated. Intracellular distribution of 5-LOX protein was detected by immunocytochemistry, and the mRNA expressions of CysLT1 and CysLT2 receptors were detected by RT-PCR. The effect of leukotriene D(4) (LTD(4)) on C6 cells was also investigated.
RESULTOGD for 4-8 h followed by recovery for 24-72 h significantly induced C6 cell injury. Neither 5-LOX inhibitors nor CysLT receptor antagonists inhibited OGD-induced injury. OGD did not induce 5-LOX translocation into the nuclear membrane. C6 cells highly expressed CysLT(2) receptor, but the expression of CysLT1receptor was much weaker; the expression was not affected by OGD. In addition, LTD(4) did not affect C6 cells significantly.
CONCLUSIONOGD can induce C6 cell injury, but 5-LOX/CysLT pathway might be not involved in OGD-induced injury.
