Establishment of animal model with B lineage acute leukemia in nude mice for evaluation of new therapeutic agents.
- Author:
Li-xia LI
1
;
Yong-min TANG
;
Wei-zhong GU
;
Hong-feng TANG
;
Bai-qin QIAN
;
Hong-qiang SHEN
;
Chun-fang LUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cyclophosphamide; Disease Models, Animal; Female; Mice; Mice, Inbred BALB C; Mice, Nude; Neoplasm Transplantation; Precursor Cell Lymphoblastic Leukemia-Lymphoma; etiology
- From: Journal of Zhejiang University. Medical sciences 2008;37(5):511-514
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an acute leukemia animal model for testing new therapeutic agents in vivo.
METHODSNude mice were intraperitoneally injected with 2 mg cyclophosphamide, 24 h later 5 x 10(6) acute B-cell leukemia Nalm-6 cells was inoculated via the tail vein, then monitored daily. When animals were paralyzed or dying, the organs including the liver, spleen, lung, heart, kidney, brain, bone marrow, pancreas, testes were removed and fixed with formalin, examined by routine histopathology.
RESULTSAfter Nalm-6 cells were inoculated the mean survival of mice were( 19.4+/-0.55)d (n=6). The paralysis of mice was followed by weight loss, bent spines, hogback, cachexia and death. Histopathological examination showed that the tumor cells infiltrated liver, spleen, kidney, lung, meninges, interior cerebrum, the liver and kidney were the most affected organs.
CONCLUSIONB lineage acute leukemia animal model has been successfully established in the nude mice, which is suitable for testing new therapeutic agents.
