Expression of Ref-1 in hippocampus CA1 area of rats with Alzheimer's disease.
- Author:
Hai-qin WU
1
;
Li YAO
;
Yun DU
;
Gui-lian ZHANG
;
Ru ZHANG
;
Jing-jie LIU
;
Ning PU
Author Information
- Publication Type:Journal Article
- MeSH: Alzheimer Disease; chemically induced; metabolism; Amyloid beta-Peptides; administration & dosage; Animals; DNA-(Apurinic or Apyrimidinic Site) Lyase; metabolism; Hippocampus; drug effects; metabolism; Male; Maze Learning; drug effects; Random Allocation; Rats; Rats, Sprague-Dawley
- From: Journal of Zhejiang University. Medical sciences 2008;37(6):629-633
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of Redox factor-1(Ref-1) in rats with Alzheimer's disease (AD).
METHODSAD rat model was established by a single injection of beta-amyloid peptide (Abeta25-35) into the lateral cerebral ventricle of rats. Y-shape maze task was conducted to check the ethology. At 4th, 7th and 14th day after injection, Ref-1 expression in hippocampus CA1 area was detected by immunohistochemistry.
RESULTThe numbers of repeated learning and error and the total reaction time in AD group were significantly higher than those in control group at 7th and 14th day after injection of Abeta25-35 (P<0.05). At 4th day after injection of Abeta25-35, the Ref-1 expression was increased with time prolongation in AD group(P<0.01).
CONCLUSIONInjection of Abeta25-35 in lateral cerebral ventricle can decrease the learning and memory abilities of rats, and results also indicate that Ref-1 expression in hippocampus may be involved in the development of AD.
