Peptide MC10 mediated PEI-beta-CyD as a gene delivery vector targeting to Her-2 receptor.
- Author:
Jun LIU
1
;
Yi-Ping HU
;
Qi-Ying JIANG
;
Dan CHEN
;
Hai YU
;
Qing-Qing WANG
;
Gu-Ping TANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Gene Targeting; Gene Transfer Techniques; Genetic Vectors; Humans; Peptides; chemistry; Polyethyleneimine; chemistry; pharmacology; Receptor, ErbB-2; genetics; beta-Cyclodextrins; chemistry
- From: Journal of Zhejiang University. Medical sciences 2009;38(1):7-14
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a novel non-viral gene delivery vector based on polyethylenimine and beta-cyclodextrin targeting to Her-2 receptor (MC10-PEI-beta-CyD).
METHODSThe PEI-beta-CyD was synthesized by low molecular weight polyethylenimine (PEI, Mw 600) cross-linked beta-cyclodextrin (beta-CyD) via N, N-carbonyldiimidazole (CDI). The chemical linker[N-succinimidy-3-(2-pyridyldithio) propionate, SPDP] was used to bind peptide MC10 (MARAKEGGGC) to PEI-beta-CyD to form the vector MC10-PEI-beta-CyD. The (1)H-NMR was used to confirm the structure of vector. The DNA condensing ability,and the particle size of MC10-PEI-beta-CyD/DNA complexes were demonstrated by gel retardation assay and electron microscope observation (TEM). Cell viability was tested by MTT assay. The transfection efficiency was determined on cultured SKOV-3, A549 and MCF-7 cells.
RESULTMC10 was linked onto PEI-beta-CyD successfully. The vector was able to condense DNA at N/P ratio of 5 and particle size was about (170 +/-35)nm. The vector showed low cytotoxicity and high transfection efficiency in cultured SKOV-3, A549 and MCF-7 cells.
CONCLUSIONA novel non-viral vector MC10-PEI-beta-CyD with low cytotoxicity and high transfection efficiency has been successfully synthesized.
