Peptide CY11 conjugated polyethylenimine-beta-cyclodextrin for gene delivery.
- Author:
Yi-Ping HU
1
;
Qi-Ying JIANG
;
Dan CHEN
;
Dan-Jun ZHAO
;
Xiao LU
;
Hai YU
;
Qing-Qing WANG
;
Jun ZHOU
;
Xiu-Rong HU
;
Gu-Ping TANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Gene Transfer Techniques; Genetic Therapy; methods; Humans; Peptide Fragments; chemistry; Polyethyleneimine; chemistry; Receptors, Fibroblast Growth Factor; chemistry; beta-Cyclodextrins; chemistry
- From: Journal of Zhejiang University. Medical sciences 2009;38(1):24-30
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a novel non-viral gene delivery vector CY11-PEI-beta-CyD and to test its gene transfection efficiency.
METHODSCY11 (CGMQLPLATWY) was conjugated to polyethylenimine-beta-cyclodextrin to form CY11-PEI-beta-CyD with a cross-linker [N-succinimidy-3-(2-pyridyldithio) propionate, SPDP]. (1)H-NMR and TGA were used to confirm the structure of vector. The DNA condensing ability of CY11-PEI-beta-CyD was investigated by gel retardation assay. Cytotoxicity of CY11-PEI-beta-CyD was determined by MTT assay and transfection efficiency was investigated in COS-7, Hela and B16 cells.
RESULTCY11 was conjugated onto PEI-beta-CyD successfully, confirmed by(1)H NMR and TGA. The novel vector effectively condensed DNA at N/P ratio of 4îIt showed low cytotoxicity up to the concentration was 160 Mgr;g/ml. The transfection efficiency was 17-fold higher than that of PEI 25 kDa at N/P ratio of 20.
CONCLUSIONThe novel vector CY11 -PEI-beta-CyD with low cytotoxic and high transfection efficiency may be used as a potential carrier for gene delivery.
