Poly-aspartamide-glutamic acid grafted low molecular weight polyethylenimine as a novel non-viral gene vector.
- Author:
Dan CHEN
1
;
Bin-Bin HE
;
Dan-Jun ZHAO
;
Qi-Ying JIANG
;
Zhong-Ren WANG
;
Jun ZHOU
;
Hai YU
;
Qing-Qing WANG
;
Gu-Ping TANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Gene Transfer Techniques; Genetic Therapy; methods; Glutamic Acid; chemistry; Humans; Peptides; chemistry; Polyethyleneimine; chemistry
- From: Journal of Zhejiang University. Medical sciences 2009;38(1):31-38
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a novel gene delivery vector with poly-aspartamide-glutamic acid and polyethylenimine as the backbone.
METHODSalpha, beta-poly-(N-2-hydroxypropyl)-D, L-aspartamide-glutamic acid (PHPAG) was synthesized and low molecular weight polyethylenimine (PEI 1.8 kDa) was grafted to form PHPAG-PEI 1800. Chemical and biological characterization of the polymer was identified.
RESULTThe polymer was confirmed by (1)H-NMR, and the molecular weight was about 1.2 x 10(4). The ability of DNA binding was showed by gel retardation assay at N/P ratio of 3. 5. MTT assay showed that the polymer was non toxic in COS-7 and A293 cell lines. In vitro test demonstrated that it had high transfection efficiency in B16 and Hela cell lines.
CONCLUSIONPHPAG-PEI 1800 was successfully synthesized,which might be a potential vector for gene delivery.
