Lentinan-graft-polyethylenimine-a novel vector for gene delivery.
- Author:
Qi-Ying JIANG
1
;
Xiao LU
;
Yi-Ping HU
;
Dan CHEN
;
Dan-Jun ZHAO
;
Jun ZHOU
;
Hai YU
;
Qing-Qing WANG
;
Gu-Ping TANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Folic Acid; chemistry; Gene Transfer Techniques; Genetic Therapy; methods; Humans; Lentinan; chemistry; Polyethyleneimine; chemistry
- From: Journal of Zhejiang University. Medical sciences 2009;38(1):39-45
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a novel vector for gene delivery with low molecular weight polyethylenimine grafted to the natural polysaccharide and conjugated to folic acid (LNT-PEI-FA).
METHODSThe properties of LNT-PEI-FA were characterized by (1)H-NMR, FT-IR and TGA, respectively. The particle size of LNT-PEI-FA/DNA complex was measured. The DNA binding ability of LNT-PEI-FA was detected by gel electrophoresis retardation assay.
RESULTThe particle size of LNT-PEI-FA/DNA complex was about 200 nm. Gel electrophoresis showed that at N/P ratio of 1.8 (W/W) the polymer was able to completely condense DNA. In vitro experiments showed a high efficiency of gene transfection in A293 and B16 cell lines.
CONCLUSIONA novel non-viral vector LNT-PEI-FA was successfully synthesized and characterized, which may be applied in gene transfection research in the future.
