Aspirin-PEI-beta-CyD as a novel non-viral vector for gene transfer.
- Author:
Zhong-Ren WANG
1
;
Dan CHEN
;
Jun ZHOU
;
Gu-Ping TANG
Author Information
- Publication Type:Journal Article
- MeSH: Aspirin; chemistry; Cell Line; Gene Transfer Techniques; Genetic Therapy; methods; Humans; Polyethyleneimine; chemistry; beta-Cyclodextrins; chemistry
- From: Journal of Zhejiang University. Medical sciences 2009;38(1):46-52
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a novel non-viral gene delivery vector based on PEI-beta-CyD as backbone modified with aspirin, and to identify its physicochemical characters.
METHODS1, 1-carbonyldiimidazole (CDI) was used to bind aspirin onto PEI-beta-CyD to form PEI-beta-CyD-ASP. (1)H-NMR, FT-IR, UV and XRD were used to confirm the polymer structure. The ability of condensation was demonstrated by gel retardation assay. MTT assay was used to test the cell viability in B16, Hela and A293 cell lines. Transfection efficiency of the polymer was tested in B16 cells.
RESULTThe structure of PEI-beta-CyD-ASP was confirmed by (1)H-NMR, FT-IR, UV and XRD, which efficiently condensed plasmid DNA at the N/P ratio of 4. The copolymer showed low cytotoxicity and high transfection efficiency in B16 cells.
CONCLUSIONThe synthesized aspirin-PEI-beta-CyD might be a potential gene delivery vector.
