Platinum tetrachloride coupled PEG-PEI as drug carrier and gene delivery vector.
- Author:
Lu ZHAO
1
;
Huan CHEN
;
Dan CHEN
;
Yi-Ping HU
;
Hai YU
;
Qing-Qing WANG
;
Gu-Ping TANG
;
Xiu-Rong HU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Drug Carriers; chemical synthesis; Gene Transfer Techniques; Genetic Therapy; methods; Humans; Platinum Compounds; chemistry; Polyethylene Glycols; chemistry; Polyethyleneimine; chemistry; Transfection
- From: Journal of Zhejiang University. Medical sciences 2009;38(1):59-66
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct a drug carrier and gene vector PEG-PEI-Pt.
METHODSPolyethyleneglycol (PEG) was coupled to polyethylenimine (PEI 600) and platinum tetrachloride; PEG-PEI-Pt complex was formed in ethanol. The complex was characterized by XRD, UV-VIS and FT-IR and the DNA condensation was tested by electrophoretic mobility shift assay. The cell viability was evaluated by MTT assay in Hela, B16, A293 and COS-7 cells and in vitro transfection efficiency was measured in A293 and B16 cells.
RESULTThe structure of PEG-PEI-Pt was characterized by XRD, UV-VIS and FT-IR. PEG-PEI-Pt complex was able to bind DNA at N/P weight ratio of 0.4:1; the complex showed cytotoxicity on Hela and B16 cells. The complex had higher transfection efficiency in A293 and B16 cells than PEI 600.
CONCLUSIONA novel drug carrier and gene vector PEG-PEI-Pt was constructed successfully.
