Effects on activity of mouse NK cells by inhibitory Ly49 receptors blockade.
- Author:
Yun XU
1
;
He HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; immunology; pharmacology; Cell Proliferation; Cells, Cultured; Female; Genes, MHC Class I; Killer Cells, Natural; immunology; Lymphocyte Culture Test, Mixed; Lymphoma; immunology; pathology; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; NK Cell Lectin-Like Receptor Subfamily A; immunology; Spleen; cytology; immunology
- From: Journal of Zhejiang University. Medical sciences 2009;38(2):130-135
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects on antitumor activity of mouse NK cells and the mixed lymphocyte reaction with blocking the interaction between the inhibitory Ly49 receptor on mouse NK cell and MHC-I molecules on target cells with monoclonal antibody.
METHODSNK cells were isolated from splenocytes of C57BL/6J mouse with immunomagnetic method. YAC-1 and M1 cells were used as target cells, the cytotoxicity of NK cells was examined with mixed lymphocyte reaction before and after treatment of anti-Ly49 monoclonal antibody.
RESULTThe cytotoxicity of NK cells to M1 cell was significantly augmented (10.7% +/- 0.5% compared with 84.4 % +/- 2.9%), and the clone-forming capacity of M1 cell was inhibited remarkably (8.2 % +/- 2.2% compared with 94.0% +/- 3.3%) with the increase of concentration of the antibody (0 approximate, equals 60 microg/ml) (P <0.05). The difference of cytotoxicity to YAC-1 cells was only found between 0 microg/ml and 60 microg/ml groups (P <0.05). In mixed lymphocyte reaction, after pretreated with antibody, the proliferation of splenocytes of BALB/C mouse was inhibited (0.398 +/-0.025 compared with 0.128 +/- 0.014), and statistical difference was observed only between antibody groups of 0 microg/ml and 60 microg/ml (P<0.05).
CONCLUSIONBlocking the inhibitory Ly49 receptor on NK cells with monoclonal antibody augments the cytotoxicity to target cells.
