Functional regulation of endothelial Myosin light chain kinase in extravascular migration of fibrosarcoma cells.
- Author:
Hua XIN
1
;
Zhen-guo HAN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Movement; drug effects; Endothelial Cells; cytology; enzymology; Fibrosarcoma; pathology; physiopathology; Humans; Myosin-Light-Chain Kinase; metabolism; physiology; Neoplasm Invasiveness; Umbilical Veins; cytology
- From: Journal of Zhejiang University. Medical sciences 2009;38(2):145-150
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the functional regulation of endothelial Myosin light chain kinase (MLCK) in extravascular migration of fibrosarcoma HT1080 cells.
METHODSAn in vitro model of fibrosarcoma cell transmigration across a monolayer of HUVEC cultured on collagen gel was applied to observe extravascular migration of HT1080 cells,and were the electrical resistance of HUVEC monolayer and endothelial MLC phosphorylation in extravascular migration of HT1080 cells.
RESULTHT1080 cells migrated through endothelial cells into collagen gel, the electrical resistance of a HUVEC monolayer was reduced and endothelial MLC phosphorylation was enhanced in extravascular migration of fibrosarcoma cells. Endothelial MLCK inhibitor (ML-7) blocked extravascular migration of HT1080 cells and inhibited reduction of electrical resistance of a HUVEC monolayer and enhancement of endothelial MLC phosphorylation in extravascular migration of HT1080 cells in a dose-dependent manner.
CONCLUSIONEndothelial MLCK regulates fibrosarcoma cell transendothelial migration through MLC phosphorylation, leading to cytoskeletal reorganization and endothelial cell constriction, then fibrosarcoma cells migrate into extravascular tissue through the gaps between endothelial cells.
