Determination of beta-estradiol, bisphenol A, diethylstilbestrol and salbutamol in human urine by GC/MS.
- Author:
Yuan-Yang WU
1
;
Wei-Xing SHI
;
Shu-Qing CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Albuterol; urine; Benzhydryl Compounds; Diethylstilbestrol; urine; Environmental Pollutants; urine; Estradiol; urine; Gas Chromatography-Mass Spectrometry; Humans; Phenols; urine
- From: Journal of Zhejiang University. Medical sciences 2009;38(3):235-241
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a GC/MS method for analysis of beta-estradiol (beta-E2), bisphenol A (BPA), diethylstilbestrol (DES) and salbutamol (SAL) in human urine.
METHODSHuman urine samples were extracted by cleanert PCX and cleanert PEP cartridges; and derivatized after dried completely. beta-E2, BPA, DES and SAL in the extracts were measured by GC/MS method with DB-5MS capillary column and EI ion-source.
RESULTThe calibration curves for beta-E2 in samples were linear over the concentration ranges of 1 approximately 300 ng ml(-1), for BPA were 1 approximately 200 ng ml(-1), for DES were 2 approximately 300 ng ml(-1) and for SAL were 0.01 approximately 1.2 microg ml(-1). The limits of detection were 0.15 ng ml(-1), 0.19 ng ml(-1), 0.23 ng ml(-1) and 1.0 ng ml(-1)for beta-E2, BPA, DES and SAL, respectively. The assay recoveries for beta-E2, BPA, DES and SAL ranged from 93.4 % approximately 110.5 %, 84.7 % approximately 104.9 %, 87.0 % approximately 105.4 % and 81.8 % approximately 96.8 %, respectively.
CONCLUSIONThe established GC/MS method can detect beta-E2, BPA, DES and SAL in urine samples simultaneously, which can be used in routine assessment and monitoring of beta-E2, BPA, DES and SAL in human body.