Effect of concanavalin A on expression of MAPEG mRNA in Balb/c mouse brain.
- Author:
Xiang-Nan ZHANG
1
;
Lou-Yang QI
;
Dan-Yan ZHU
;
Yi-Jia LOU
Author Information
- Publication Type:Journal Article
- MeSH: 5-Lipoxygenase-Activating Proteins; Animals; Brain; metabolism; Carrier Proteins; genetics; metabolism; Concanavalin A; toxicity; Cyclosporine; pharmacology; Eicosanoids; metabolism; Glutathione; metabolism; Glutathione Transferase; genetics; metabolism; Intramolecular Oxidoreductases; genetics; metabolism; Male; Membrane Proteins; genetics; metabolism; Mice; Mice, Inbred BALB C; Prostaglandin-E Synthases
- From: Journal of Zhejiang University. Medical sciences 2009;38(3):255-259
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the gene expression of MAPEG in the cortex of concanavalin A (Con A)-induced mouse immune inflammatory model and the effect of cyclosporine A (Cs A).
METHODSMale Balb/c mouse immune inflammation model was developed by intravenous injection of Con A (20 mg/kg). Cs A (150 mg/kg) was intravenously infected prior to Con A administration. The MAPEG expressions were determined by RT-PCR.
RESULTmGST1, mGST3, LTC(4)S, FLAP and mPGES-1 were detected by RT-PCR but not mGST2. Eight hours after Con A treatment, mGST1 level was up-regulated to 1.2 approximately 1.5 folds of control with or without Cs A treatment. mGST3ìLTC(4)S, FLAP and mPGES-1 mRNA levels were not influenced by Con A administration.
CONCLUSIONImmune mechanism may be not involved in mGST1 up-regulation in this model and Con A does not alter arachidonic acid metabolism in cortex.
