Preparation and identification of monoclonal antibodies to staphylococcal enterotoxin I.
- Author:
Jun-Ying XU
1
;
Ding DING
;
Hong-Ying SUN
;
Shu-Qing CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; biosynthesis; immunology; Enterotoxins; immunology; Hybridomas; secretion; Immunoglobulin G; biosynthesis; immunology; Mice; Mice, Inbred BALB C; Spleen; cytology; Staphylococcus aureus; immunology
- From: Journal of Zhejiang University. Medical sciences 2009;38(3):265-270
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare and identify monoclonal antibodies against staphylococcal enterotoxin I (SEI).
METHODSSpleen cells obtained from mice immunized with the SEI protein were fused with the myeloma cells (SP2/0). Hybridomas were screened by enzyme-linked immunosorbent assay (ELISA) and the stable monoclonal hybridomas were isolated by limiting dilution at least three times. The characters of purified monoclonal antibodies were identified by indirect ELISA and Western blotting.
RESULTThe monoclonal antibodies secreted by two hybridomas 8F7 and D8 belonged to IgG(2b) and IgG(1) subtypes. Both had high titer and specificity with no cross reaction to SEG, SEE and SEC.
CONCLUSIONThe monoclonal antibodies against SEI has been successfully prepared and identified in this study.
