Autocrine effect of vascular endothelial growth factor on the proliferation of HaCaT cells.
- Author:
Xiao-hong YANG
1
;
Xiao-yong MAN
;
Sui-qing CAI
;
Chun-ming LI
;
Jiong ZHOU
;
Min ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Autocrine Communication; Cell Line; Cell Proliferation; drug effects; Dose-Response Relationship, Drug; Epidermis; cytology; Humans; Keratinocytes; cytology; Skin; cytology; Vascular Endothelial Growth Factor A; pharmacology
- From: Journal of Zhejiang University. Medical sciences 2009;38(4):338-342
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the autocrine effect of vascular endothelial growth factor (VEGF) on epidermal keratinocytes HaCaT cells.
METHODSCultured HaCaT cells were treated with various concentrations of VEGF(165) (0,1,5,10,25,50,100 ng/ml) or Avastin (0,0.063,0.125,0.25,0.50,1.0,2.0 mg/ml) in vitro. HaCaT cell proliferation was determined by MTT assay and the cell migration was measured by migration assay. The effect of VEGF(165) (10 ng/ml) on phosphorylation of ERK1/2 was detected in HaCaT cells pretreated or not pretreated with Avastin (0.5 mg/ml).
RESULTSVEGF enhanced the proliferation and migration of HaCaT cells in a dose-dependent manner, while Avastin inhibited the effects of VEGF also in a dose-dependent manner. VEGF(165) (10 ng/ml) induced the phosphorylation of ERK1/2 in HaCaT cells,but which was blocked by Avastin (0.5 mg/ml).
CONCLUSIONVEGF enhanced the proliferation and migration of HaCaT cells in a dose-dependent manner, while Avastin inhibited the effects of VEGF also in a dose-dependent manner. VEGF(165) (10 ng/ml) induced the phosphorylation of ERK1/2 in HaCaT cells,but which was blocked by Avastin (0.5 mg/ml).
