Effects of 8-Methoxypsoralen on intracellular Ca(2+)i and cytoskeleton actin organization in human melanocytes in vitro.

Author: Xian-qi ZHANG ¹ ; Min ZHENG ; Kuan-hou MOU ; Jie FENG
Author Information

1. Department of Dermatology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China.
Publication Type:Journal Article
MeSH: Actins; biosynthesis; genetics; Calcium; metabolism; Cell Movement; drug effects; Cells, Cultured; Cytoskeletal Proteins; biosynthesis; genetics; Humans; Melanocytes; cytology; drug effects; metabolism; Methoxsalen; pharmacology; Skin; cytology
From: Journal of Zhejiang University. Medical sciences 2009;38(4):348-351
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the effects of 8-methoxypsoralen on human melanocytes [Ca(2+)]i and cytoskeleton actin organization in vitro.
METHODSHuman melanocytes were obtained from normal foreskins. Laser confocal microscope was employed to measure [Ca(2+)]i and rhodamine-conjugated phalloidin was used to visualize the cytoskeleton actin.
RESULTS8-methoxypsoralen increased [Ca(2+)]i and induced organization of actin stress fiber cytoskeleton.
CONCLUSION8-methoxypsoralen might influence the migration of melanocytes by increasing the intracellular free Ca(2+) concentration and cytoskeleton actin reorganization.