Preparation and identification of a polyclonal antibody against novel cysteinyl leukotriene receptor GPR17.
- Author:
Ling-ling QI
1
;
Yun-bi LU
;
Wen-zhen SHI
;
Chun-zhen ZHAO
;
Yong-mei ZHANG
;
Li-ping CHEN
;
Li-hui ZHANG
;
San-hua FANG
;
Jian-fang BAO
;
Jian-gen SHEN
;
Er-qing WEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; biosynthesis; immunology; Enzyme-Linked Immunosorbent Assay; Humans; Rabbits; Rats; Receptors, G-Protein-Coupled; immunology; Receptors, Leukotriene; immunology
- From: Journal of Zhejiang University. Medical sciences 2009;38(4):357-361
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare and identify a polyclonal antibody (pAb) against GPR17, a novel cysteinyl leukotriene receptor.
METHODSRabbits were immunized with KLH-coupled GPR17 peptide to prepare the pAb. The titer of the pAb in rabbit plasma was detected by indirect ELISA, and the specificity of the pAb was tested by antigen blockade. GPR17 tissue distribution was detected by Western blot with the pAb.
RESULTSThe pAb showed a titer as high as 1:16 364,and was not cross-reacted with the antigens of CysLT(1) and CysLT(2) receptors. A higher expression of GPR17 in the rat brain and heart was detected using the newly prepared pAb. The molecular weigh of GPR17 protein was about 43 kD.
CONCLUSIONThe prepared GPR17 pAb has high sensitivity and specificity,and can be used in Western blot for detecting GPR17.
