The immunization effects of HBV core antigen and surface antigen fusion protein in mice.
- Author:
Lei LI
1
;
Yong-jun TIAN
;
Hong SHEN
;
Xi-ping ZHAO
;
Dong-liang YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; Hepatitis B; genetics; immunology; prevention & control; Hepatitis B Antibodies; blood; Hepatitis B Core Antigens; genetics; immunology; Hepatitis B Surface Antigens; genetics; immunology; Hepatitis B Vaccines; genetics; immunology; Hepatitis B virus; genetics; immunology; Mice; Mice, Inbred BALB C; Plasmids; genetics; Recombinant Fusion Proteins; biosynthesis; genetics; immunology; T-Lymphocytes; immunology; Viroids; genetics
- From: Chinese Journal of Hepatology 2009;17(4):275-279
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the immunization effects of HBV core antigen and surface antigen fusion protein.
METHODSThe DNA fragments encoding HBsAg 100-162 aa; HBcAg 1-78 aa and HBcAg 83-144 aa were PCR-amplified, and then cloned into pcDNA3 plasmid. The chimeric gene was subcloned into the prokaryotic vector, pRSET-B. The E.coli expressed recombinant protein purified. BALB/c mice were immunized with recombinant protein or eukaryotic expression plasmid, humoral response and cellular response were examined.
RESULTSThe plasmid containing the chimeric gene of HBsAg and HBcAg induced effective anti-HBs antibody response and strong HBcAg specific lymphocyte proliferative response, but could not induce anti-HBc antibody response. Fusion protein induced strong anti-HBs and anti-HBc antibody response, and it also caused significant HBcAg specific lymphocyte proliferation. Compared to the recombinant fusion protein, the plasmid containing the chimeric gene of HBsAg and HBcAg can induce more effective cellular response but weaker humoral response.
CONCLUSIONCompared to the recombinant fusion protein, the plasmid containing the chimeric gene of HBsAg and HBcAg is a more effective vaccine.
