The effect of hepatitis B virus X protein on the c-met promoter activity in HepG2 cells.
- Author:
Bin XIE
1
;
Chun TANG
;
Ping CHEN
;
Yuan-bin GOU
;
Jing XIAO
;
Hu DU
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Western; Carcinoma, Hepatocellular; genetics; metabolism; pathology; Gene Expression Regulation; Genes, Viral; Hep G2 Cells; Hepatitis B virus; genetics; Humans; Plasmids; Polymerase Chain Reaction; Promoter Regions, Genetic; genetics; Proto-Oncogene Proteins c-met; metabolism; Trans-Activators; genetics; metabolism; Transcription Factors; metabolism; Transfection
- From: Chinese Journal of Hepatology 2009;17(4):292-296
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo confirm the effect of hepatitis B virus X (HBx) protein on the c-met promoter activity in HepG2 cells.
METHODThe expression of c-met protein was detected by western blot in HBx-transfected HepG2 cells, the human c-met promote activity was checked by luciferase assay using five different constructs with deletion or point mutation.
RESULTSHBx protein stimulated the expression of the c-met in HepG2 cells. The enhanced expression of c-met in HBx-transfected cells was mediated by the activation of AP-2 and SP-1 transcriptional activity at the c-met promoter region (-183bp--100bp), HBx increased the invasiveness of HepG2 cells as determined by Matrigel invasion assay.
CONCLUSIONThese results suggests that HBx induces the expression of c-met through the activation of AP-2 and SP-1 activity at the promoter region; in addition, our data indicate that HBx stimulates the invasive potential of HepG2 cells.
