Hepatitis B virus P22(e) inhibit hepatocyte apoptosis via nuclear factor kappa B.
- Author:
Zhi-hong DIAO
1
;
Ming-xia ZHANG
;
You-fu ZHU
;
Yu-ling SHI
;
Jin-lin HOU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Carcinoma, Hepatocellular; metabolism; Hep G2 Cells; Hepatitis B Core Antigens; metabolism; Hepatitis B virus; genetics; Humans; Leupeptins; pharmacology; Liver Neoplasms; metabolism; NF-kappa B; antagonists & inhibitors; metabolism; Plasmids; Signal Transduction; drug effects; Transfection; Viral Core Proteins; metabolism
- From: Chinese Journal of Hepatology 2009;17(5):359-362
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo test whether nuclear factor kappa B plays an important role in the apoptosis-inhibitory effect of hepatitis B virus (HBV) P22(e) protein.
METHODSHepG2 cells were transfected with recombination plasmid pEGFP-HBVP22(e). The Act-D and TNF alpha were used to induce apoptosis. NF-kappa B inhibitor ALLN were used to inhibit the signaling pathway. The activation of NF-kappa B was EMSA, and the nulear translocation of NF-kappa B was determined by immuno-staining.
RESULTSLaser scanning confocal microscopy and EMSA indicated that HBV P22(e) protein enhanced the nuclear translocation of NF-kappa B after apoptosis induction. ALLN treatment inhibited the nuclear translocation of NF-kappa B, and blocked the apoptosis-inhibiting effect of HBV P22(e) protein.
CONCLUSIONThis study indicates that HBV P22(e) protein inhibits apoptosis of hepatocyte via the NF-kappa B signaling pathway.
