Role of IL-6/STAT3 in rat cholangiocyte proliferation induced by lipopolysaccharide.
- Author:
Li-ping CHEN
1
;
Ye-yong QIAN
;
Zhou-li LI
;
Hong-wei BAI
;
Ming CAI
;
Bing-yi SHI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; administration & dosage; Bile Ducts, Intrahepatic; cytology; Cell Proliferation; Epithelial Cells; cytology; physiology; Immunohistochemistry; Interleukin-6; genetics; immunology; metabolism; Lipopolysaccharides; blood; pharmacology; Liver; metabolism; Male; RNA, Messenger; genetics; metabolism; Random Allocation; Rats; Rats, Wistar; STAT3 Transcription Factor; metabolism; Signal Transduction
- From: Chinese Journal of Hepatology 2009;17(5):374-377
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether lipopolysaccharide (LPS) stimulates cholangiocyte proliferation via the IL-6/STAT3 pathway in vivo.
METHODSRats were randomized into three groups: LPS group (injected intravenously with LPS 2.5 mg/kg), anti-IL-6 group (injected intravenously with anti-IL-6 0.5 mg/kg 1hr after LPS injection), and control group. At 6, 12, 24, 48 and 72 h after LPS injection, LPS concentration in plasma was detected by kinetic turbidimetric limulus test. IL-6 concentrations in liver homogenate was determinded by ELISA, cholangiocyte proliferation was checked by immunohistochemistry, expression of IL-6 mRNA was quantified by real-time RT-PCR, the level of phophorylated-STAT3 (P-STAT3) protein was analyzed by western blotting.
RESULTSCholangiocytes responded to LPS by a marked increase in cell proliferation, IL-6 secretion and P-STAT3 expression. Anti-IL-6 neutralizing antibody inhibited LPS-induced cholangiocytes proliferation, and decreased levels of IL-6 and p-STAT3.
CONCLUSIONSLPS promotes cholangiocyte proliferation through the IL-6/STAT3 pathway.
