Interference of osteopontin expression inhibits the invasion and metastasis of human hepatocellular carcinoma cell lines.
- Author:
Fan LIN
1
;
Yu-yuan LI
;
Jin-tang XIA
;
Min-jie WEN
;
Yue-yuan LAI
;
Wen-song CAI
;
Zhao-feng WU
;
Shao-feng FAN
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; genetics; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; Gene Expression Regulation, Neoplastic; Genetic Vectors; Humans; Liver Neoplasms; genetics; metabolism; pathology; Neoplasm Invasiveness; prevention & control; Neoplasm Metastasis; prevention & control; Osteopontin; antagonists & inhibitors; genetics; metabolism; RNA, Messenger; genetics; metabolism; RNA, Small Interfering; genetics; Transfection
- From: Chinese Journal of Hepatology 2009;17(6):422-425
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of osteopontin (OPN) on the invasion and metastasis of human hapatocellular carcinoma (HCC).
METHODSHCC cell lines (HCC-LM3) were transfected with the chemically synthesized small interfering RNA (siRNA). Real-time PCR and Western blot were used to quantify the mRNA and OPN protein levels. The malignant phenotypes including cellular growth, colony formation and invasion capability of the HCC cells were analyzed.
RESULTSThe OPN mRNA and proteins levels were decreased by 75% and 80% in OPN siRNA treated cells. Colony formation and migratory capability were reduced in OPN siRNA treated cells (P < 0.05).
CONCLUSIONThe specific siRNA is able to reduce the OPN expression at both the mRNA and protein levels and significantly inhibits the invasiveness of HCC cells.
