Insertion of TC motif into hepatitis B virus core protein c/e1 site does not affect the expression of S and e antigen.
- Author:
Yuan-yuan LIN
1
;
Xiao-ming CHENG
;
Yu-hu SONG
;
Ying CHANG
;
Jin-jian YAO
;
Ju-sheng LIN
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Blotting, Western; Enzyme-Linked Immunosorbent Assay; Genetic Vectors; genetics; metabolism; Hep G2 Cells; Hepatitis B Core Antigens; genetics; metabolism; Hepatitis B Surface Antigens; metabolism; Hepatitis B e Antigens; metabolism; Hepatitis B virus; genetics; Humans; Mutagenesis, Insertional; Recombinant Proteins; genetics; metabolism; Transfection; Viral Core Proteins; genetics; metabolism; Viral Proteins; genetics; metabolism; Virus Replication
- From: Chinese Journal of Hepatology 2009;17(8):585-588
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether insertion of TC motif into hepatitis B virus (HBV) core protein c/e1 site affects the expression of S and e antigen.
METHODSDifferent oligonucleotides encoding TC motif were inserted into the c/e1 site of the core gene of a 1.3 copy wild-type HBV genome vector. HepG2 cells were divided into several groups of cells to transiently transfect with the wild-type and mutant HBV vectors, respectively. In each group, the expression level of core protein inside cells was detected by western blotting, and the levels of S and e antigen in culture medium were analyzed by ELISA assay.
RESULTSWestern blotting showed that these TC-tagged core proteins were expressed at similar level of wild-type one. ELISA assay indicated that the level of S and e antigen in culture medium of different groups were not significantly different.
CONCLUSIONInsertion of TC motif into HBV core protein c/e1 site does not interference with the expression of viral protein encoded by HBV genome.
