Analysis on the expression of the stem cells related genes in hepatocellular carcinoma cell lines.
- Author:
Can ZOU
1
;
Fang-jun YUAN
;
Wen-bo ZHOU
;
Ling HUANG
;
Ju WANG
;
You-shun ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; metabolism; pathology; DNA Methylation; Gene Expression Regulation, Neoplastic; Hep G2 Cells; Homeodomain Proteins; genetics; metabolism; Humans; Liver Neoplasms; metabolism; pathology; Nanog Homeobox Protein; Octamer Transcription Factor-3; genetics; metabolism; RNA, Messenger; genetics; metabolism; Receptors, G-Protein-Coupled; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; methods; SOXB1 Transcription Factors; genetics; metabolism; Signal Transduction; Smoothened Receptor; Stem Cells; drug effects; metabolism; Tretinoin; pharmacology; Wnt Proteins; genetics; metabolism; beta Catenin; genetics; metabolism
- From: Chinese Journal of Hepatology 2009;17(8):599-602
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of Oct4, Sox2, Nanog, SMO, beta-Catenin and Wnt5b mRNA in four hepatocellular carcinoma cell lines of SMMC-7721, Bel-7402, HepG2, MHCC-97 and normal hepatocellular cell line of L02, and to compare the response of these cell lines to all-trans retinoic acid.
METHODSRT-PCR was used to detect expression of Oct4, Sox2, Nanog, SMO, beta-Catenin and Wnt5b mRNA in four hepatocellular carcinoma cell lines and normal hepatocellular cell line. Real time-PCR was used to quantify the expression of the genes.
RESULTSThere are different levels of expression of the stem cell-related gene in hepatocellular carcinoma cell lines and control cell line (P less than 0.05). There are significant differences in HepG2 and L-02 for the response to all-trans retinoic acid (P less than 0.05).
CONCLUSIONSThe stem cell-related genes are differentially expressed in different hepatocellular carcinoma cell lines.
