Study on pharmacokinetics and tissue distribution of the isocorydine derivative (AICD) in rats by HPLC-DAD method.
10.1016/j.apsb.2015.03.012
- Author:
Yali CHEN
1
,
2
;
Qian YAN
3
;
Mei ZHONG
3
;
Quanyi ZHAO
4
;
Junxi LIU
3
;
Duolong DI
3
;
Jinxia LIU
5
Author Information
1. Institute of Medicinal Chemistry, School of Pharmacy, Lanzhou University, Lanzhou 730000, China
2. Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
3. Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China.
4. Institute of Medicinal Chemistry, School of Pharmacy, Lanzhou University, Lanzhou 730000, China.
5. Institute of Biology, Gansu Academy of Sciences, Lanzhou 730000, China.
- Publication Type:Journal Article
- Keywords:
8-Acetamino-isocorydine;
AICD, 8-acetamino-isocorydine;
AUC, area under concentration-time curve;
Alkaloids;
F, absolute bioavailability;
HPLC-DAD, high-performance liquid chromatography with diode-array detection;
HPLC-UV, high-performance liquid chromatography coupled with ultraviolet detection;
High-performance liquid chromatography with diode-array detection;
ICD, isocorydine;
IS, internal standard;
LC-ESI-MS/MS, high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry;
LLE, liquid-liquid extraction;
LLOQ, lower limit of quantification;
LOD, limit of detection;
Pharmacokinetics;
QC, quality control;
RE, relative error;
RP, reverse phase;
RSD, relative standard deviation;
SD, standard deviation.;
Tissue distribution
- From:
Acta Pharmaceutica Sinica B
2015;5(3):238-245
- CountryChina
- Language:English
-
Abstract:
A simple and effective high-performance liquid chromatography with diode-array detection method coupled with a liquid-liquid extraction pretreatment has been developed for determining the pharmacokinetics and tissue distribution of a novel structurally modified derivative (8-acetamino-isocorydine) of isocorydine. According to the in vivo experiments data calculations by DAS 2.0 software, a two-compartment metabolic model was suitable for describing the pharmacokinetic of 8-acetamino-isocorydine in rats. 8-Acetamino-isocorydine was absorbed well after oral administration, and the absolute bioavailability was 76.5%. The half-life of 8-acetamino-isocorydine after intravenous and oral administration was 2.2 h and 2.0 h, respectively. In vivo, 8-acetamino-isocorydine was highly distributed in the lungs, kidney and liver; however, relatively little entered the brain, suggesting that 8-acetamino-isocorydine could not easily pass through the blood brain barrier. Our work describes the first characterization of the pharmacokinetic parameters and tissue distribution of 8-acetamino-isocorydine. The acquired data will provide useful information for the in vivo pharmacology of 8-acetamino-isocorydine, and can be applied to new drug research.
