Simultaneous determination of 3-chlorotyrosine and 3-nitrotyrosine in human plasma by direct analysis in real time-tandem mass spectrometry.
10.1016/j.apsb.2015.07.004
- Author:
Yuqiao SONG
1
;
Jie LIAO
1
;
Cheng ZHA
1
;
Bin WANG
1
;
Charles C LIU
2
Author Information
1. Medical Experiment and Analysis Center of PLA General Hospital, Beijing 100853, China.
2. ASPEC Technologies Limited Beijing, Beijing, 100102, China.
- Publication Type:Journal Article
- Keywords:
3-Chlorotyrosine;
3-Nitrotyrosine;
DART–MS/MS;
Determintion;
Human plasma
- From:
Acta Pharmaceutica Sinica B
2015;5(5):482-486
- CountryChina
- Language:English
-
Abstract:
A novel method for the simultaneous determination of 3-nitrotyrosine (NT) and 3-chlorotyrosine (CT) in human plasma has been developed based on direct analysis in real time-tandem mass spectrometry (DART-MS/MS). Analysis was performed in the positive ionization mode using multiple reaction monitoring (MRM) of the ion transitions at m/z 216.2/170.1 for CT, m/z 227.2/181.1 for NT and m/z 230.2/184.2 for the internal standard, d (3)-NT. The assay was linear in the ranges 0.5-100 μg/mL for CT and 4-100 μg/mL for NT with corresponding limits of detection of 0.2 and 2 μg/mL. Intra- and inter-day precisions and accuracies were respectively <15% and ±15%. Matrix effects were also evaluated. The method is potentially useful for high throughput analysis although sensitivity needs to be improved before it can be applied in clinical research.
