Effect of isorhmnetin on circadian rhythms of DNA synthesis and expression of c-myc gene in Eca-109 cells of human oesophageal cancer.
- Author:
Chunlei YANG
1
;
Tao PENG
;
Yi QU
;
Dachang TAO
;
Zhengrong WANG
;
Bin ZHU
Author Information
1. Department of Medical Cell Biology, Life Science College, Sichun University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Circadian Rhythm;
drug effects;
DNA;
biosynthesis;
Esophageal Neoplasms;
genetics;
metabolism;
pathology;
Flavonols;
pharmacology;
Humans;
Proto-Oncogene Proteins c-myc;
biosynthesis;
genetics;
Quercetin;
analogs & derivatives;
Tumor Cells, Cultured
- From:
Journal of Biomedical Engineering
2005;22(6):1227-1230
- CountryChina
- Language:Chinese
-
Abstract:
This study was focused on the circadian rhythms of DNA synthesis and the expression of c-myc gene in untreated and treated Eca-109 cells in human oesophageal cancer with isorhmnetin. The circadian rhythms of 3H-TdR incorporation and expression of c-myc gene in untreated and treated Eca-109 cells were measured by 3H-thymidine uptake assay and flow cytometry. The data collected were analyzed by ANOVA and Cosinor method. DNA synthesis and expression of c-myc gene in untreated group varied according to circadian time with statistical significance, the distribution curves of both DNA synthesis and the expression level of c-myc were fit for cosinor changes. The circadian rhythms of DNA synthesis and circadian parameters of c-myc expression in treated Eca-109 cells changed. The circadian parameters of DNA synthesis and expression level of c-myc varied after treatment by isorhmnetin. The effects of isorhmnetin on cell proliferation and c-myc expression reached the highest level from 20: 00 to 0: 00. The results provide a guidance for instituting the chemotherapy and chronotherapy of human tumors, when isorhmnetin is for use as anti-cancer agent.
