Studies on the preparation and characterization of immobilized neutral protease by carboxymethyl chitosan microsphere.
- Author:
Zhitao JIN
1
;
Guohua CHEN
;
Xiaoyun LIU
;
Yu ZHAO
;
Yao CHEN
;
Congjie GAO
Author Information
1. Department of Chemistry and Chemical Engineering of Ocean University of China, Qingdao 266003, China.
- Publication Type:Journal Article
- MeSH:
Bacterial Proteins;
chemistry;
Chitosan;
analogs & derivatives;
pharmacology;
Endopeptidases;
chemistry;
Enzymes, Immobilized;
chemical synthesis;
chemistry;
Glutaral;
pharmacology;
Microspheres
- From:
Journal of Biomedical Engineering
2006;23(1):97-101
- CountryChina
- Language:Chinese
-
Abstract:
AS1. 398 neutral protease was immobilized onto carboxymethyl chitosan with glutaraldehyde as cross-linking agent. The effects of pH, time of cross-linking, amount of cross-linking agent and the ratio of enzyme to carrier on the activity of the immobilized enzyme were demonstrated, and the optimum immobilization condition of AS1. 398 neutral protease was established. Also studied was the characterization of immobilized enzyme,including pH, temperature, Km and the stability of storage.
