Dose and long-term effect of hIGF-1 injection for erectile dysfunction in aged rats.
- Author:
Xiao-Yong PU
1
;
Xing-Huan WANG
;
Huai-Peng WANG
;
Wei-Cheng GAO
;
Zhong-Hua YANG
;
Shi-Lin LI
;
Chun-Hua DENG
;
Xiang-Ming MAO
Author Information
- Publication Type:Journal Article
- MeSH: Aging; physiology; Animals; Dose-Response Relationship, Drug; Erectile Dysfunction; physiopathology; therapy; Genetic Therapy; methods; Insulin-Like Growth Factor I; genetics; physiology; Male; Penis; metabolism; physiopathology; Plasmids; administration & dosage; genetics; RNA, Messenger; genetics; metabolism; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Time Factors
- From: National Journal of Andrology 2008;14(8):690-694
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the best dose and the long-term effect of the human insulin-like growth factor-1 (hIGF-1) gene injection into the penis of aged rats.
METHODSIncluded in this study were 10 young (4 months old) and 40 aged (24 months old) Sprague-Dawley male rats, the latter equally divided into a PBS control and a 10 microg, a 100 microg and a 1 000 microg hIGF-1 injection group. Electrical stimulation was conducted 4 and 8 weeks after hIGF-1 injection into the penile corpus cavernous of the rats to detect the intracavernous pressure (ICP) and mean arterial pressure (MAP). Dose - and time -associated therapeutic results were analyzed and the mRNA expression of hIGF-1 determined by RT - PCR.
RESULTSICP, MAP and total ICP were significant decreased by electrical stimulation in the aged rats as compared with the young ones (P < 0.05), statistically increased in the three hIGF-1 dose groups in comparison with the PBS controls (P < 0.05), and showed no obvious difference between the young rats and the latter two dose groups at 4 and 8 weeks. Although less obvious effect was achieved in the 10 microg group than in the young rats, the therapeutic result was still of significance. The mRNA expression of the hIGF-1 gene was confirmed in all the hIGF-1 treated rats.
CONCLUSIONThe hIGF-1 therapy can improve erectile function in aged rats, 100 microg suffices for effective erection and the effect may last at least 8 weeks for a single dose.