Isolation of Id1 interaction protein in human prostate cancer.
- Author:
Xiao-Ling YU
1
;
Jun-Xia XIE
Author Information
- Publication Type:Journal Article
- MeSH: Activating Transcription Factor 3; analysis; metabolism; Humans; Inhibitor of Differentiation Protein 1; genetics; isolation & purification; metabolism; Male; Prostatic Neoplasms; genetics; metabolism; RNA; Reverse Transcriptase Polymerase Chain Reaction
- From: National Journal of Andrology 2008;14(9):796-799
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo isolate the inhibitor of DNA binding 1 (Id1) interaction protein and to determine the role and action mechanism of Id1 in human prostate cancer.
METHODSThe expression vector pET-28a/Id1 was established and used as a bait to prey the interaction protein by pull-down assay.
RESULTSA clear interaction protein band was observed by SDS-PAGE, which was found to be activating transcription factor 3 (ATF3) by Western blotting.
CONCLUSIONId1 may play a role in human prostate cancer by interacting with ATF3.
