Aberrant expression of sperm protein 17 enhances migration of ovarian cancer cell line HO-8910.
- Author:
Qun LIU
1
;
Fang-Qiu LI
;
Yan-Ling HAN
;
Li-Kui WANG
;
Ya-Yi HOU
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Neoplasm; genetics; Antigens, Surface; genetics; Biomarkers, Tumor; genetics; Carrier Proteins; genetics; Cell Line, Tumor; Cell Movement; Female; Gene Expression Regulation, Neoplastic; Humans; Male; Ovarian Neoplasms; metabolism; pathology; Transfection
- From: National Journal of Andrology 2008;14(11):982-986
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of aberrant expression of sperm protein 17 (Sp17) on the migration of the ovarian cancer cell line HO-8910.
METHODSThe recombinant plasmid pEGFP-Sp17 containing Sp17 and enhanced green fluorescent protein gene was transfected into the human ovarian cancer cell line HO-8910 with Lipofectamine 2000. The expression of Sp17 was examined by RT-PCR and Western blot, and the cell migratory capability detected by Transwell chamber assays.
RESULTSSp17 was expressed as a fusion protein with EGFP after transfected. There was a significant difference in the migratory cell number of the transfected and the control cells (156.6 +/- 14.9/HP vs 39.3 +/- 8.53/HP, P < 0.05).
CONCLUSIONThe aberrant expression of Sp17 greatly enhances the migration of ovarian cancer cells.
