Expressions of MMP-2 and COX-2 mRNA in bladder transitional cell carcinoma and their correlation.
- Author:
He-Peng ZHANG
1
;
Bin YU
;
Xu-Dong ZHENG
;
Hua-Jie HU
;
Zhi-Bin GAO
;
Li LI
;
Li-Fang ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Carcinoma, Transitional Cell; metabolism; pathology; Cyclooxygenase 2; biosynthesis; genetics; Female; Humans; Matrix Metalloproteinase 2; biosynthesis; genetics; Middle Aged; Neoplasm Staging; RNA, Messenger; genetics; Urinary Bladder Neoplasms; metabolism; pathology
- From: National Journal of Andrology 2008;14(11):1011-1014
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the levels of MMP-2 and COX-2 mRNA in bladder transitional cell carcinoma tissues and explore their relationship.
METHODSWe enrolled in this study 42 patients with bladder transitional cell carcinoma, including Ta-T1 (n = 18), T2-T4 (n = 24), G1 (n = 12), G2 (n = 19), G3 (n = 11), metastasis (n =26) and non-metastasis (n = 16). Another 5 cases of normal bladder tissues were taken as controls, and the levels of MMP-2 and COX-2 mRNA were detected by RT-PCR.
RESULTSThe relative expressions of COX-2 mRNA were 1.038 +/- 0. 484 in Ta-T1, 1.489 +/- 0.584 in T2-T4, 0.920 +/- 0.442 in G1, 1.338 +/- 0.584 in G2 and 1.632 +/- 0.515 in G3, all significantly higher than that of the controls (0.460 +/- 0.224, P < 0.05). And the corresponding relative levels of MMP-2 mRNA were 1.107 +/- 0.384, 1.604 +/- 0.425, 0.971 +/- 0.370, 1.445 +/- 0.378 and 1.755 +/- 0.387, also significantly higher than that of the latter group (0.423 +/- 0.227, P < 0.05). The COX-2 and MMP-2 mRNA levels in the tumor tissues with and without metastasis were 1.591 +/- 0.455 vs 0.815 +/- 0.430 and 1.676 +/- 0.339 vs 0.927 +/- 0.228, (P < 0.01), respectively, with a positive correlation between the mRNA level of COX-2 and that of MMP-2 (r = 0. 703, P < 0.01).
CONCLUSIONMMP-2 and COX-2 mRNA are highly expressed in bladder transitional cell carcinoma tissues and their expressions are positively correlated with the degree of malignancy. MMP-2 and COX-2 might play a synergetic role in the pathogenesis and progression of bladder transitional cell carcinoma.
