Growth factors promote the survival and proliferation of human spermatogonium stem cells in vitro.
- Author:
Xue-hua GUO
1
;
Yong LIN
;
Ye-ling WANG
;
Lian-wen ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Proliferation; drug effects; Cell Survival; drug effects; Cells, Cultured; Fibroblast Growth Factor 2; pharmacology; Humans; Intercellular Signaling Peptides and Proteins; pharmacology; Leukemia Inhibitory Factor; pharmacology; Male; Spermatogonia; cytology; drug effects; Stem Cell Factor; pharmacology; Stem Cells; cytology; drug effects
- From: National Journal of Andrology 2008;14(10):876-878
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of growth factors on the survival and proliferation of human spermatogonial stem cells (SSCs) in vitro.
METHODSSSCs were treated with the growth factors SCF, LIF and bFGF added to the culture, each at the concentrations of 0, 5, 10 and 20 microg/L and repeated three times. The survival time and proliferation rate of the cells were determined every 8-12 hours and their morphological features observed with the light microscope and electron microscope.
RESULTSThe survival time and proliferation rate of the SSCs were significantly increased in the treated groups as compared with the control (P < 0.05).
CONCLUSIONThe growth factors SCF, LIF and bFGF can promote the survival and proliferation of SSCs in vitro.
