Loss of heterozygosity on chromosome 10 is associated with bone metastasis of human prostate cancer.
- Author:
Zhu HOU
1
;
Yong LUO
;
Yong-guang JIANG
;
Ming-chuan LI
;
Yun-hua LIN
;
Yu-ping ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Bone Neoplasms; genetics; secondary; Chromosomes, Human, Pair 10; Comparative Genomic Hybridization; Humans; Loss of Heterozygosity; Male; Middle Aged; Neoplasm Metastasis; genetics; Prostatic Neoplasms; genetics; pathology
- From: National Journal of Andrology 2008;14(10):879-883
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the cytogenetic mechanism of bone metastasis of human prostate cancer (PCa).
METHODSWe analyzed chromosome variation by comparative genomic hybridization in 18 patients with prostate cancer to determine the chromosome variants associated with bone metastasis, and focused on 7 microsatellite sites on chromosome 10 for the detection of the loss of heterozygosity (LOH) by PCR-based microsatellite polymorphism analysis.
RESULTSIn the 11 samples with bone metastasis, the variation rate of chromosome 10 was 90.9% (10/11), significantly higher than that of the others (P < 0.01). A much higher LOH frequency was observed at the 7 microsatellite loci on chromosome 10 and the highest located in 10q24. 2-q25.3 (D10S1693-D10S587) in the PCa patients with bone metastasis.
CONCLUSIONThere is a high-frequency LOH region in 10q24. 2-q25.3 (D10S1693-D10S587) on chromosome 10 in PCa patients with bone metastasis, which may be potentially involved in PCa progression and specific bone metastasis.
