Diagnostic value for detection of aspergillus ribosomal RNA in fungal rhinosinusitis with hybridization in situ.
- Author:
Yuan LI
1
;
Yang LIU
;
Na-Ya WANG
;
Da-Zhang YANG
;
Qiu-Hang ZHANG
;
Yue WANG
;
Jie LUO
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aspergillosis; diagnosis; Aspergillus; genetics; isolation & purification; Female; Humans; In Situ Hybridization; Male; Middle Aged; RNA, Fungal; isolation & purification; RNA, Ribosomal, 18S; isolation & purification; Sinusitis; diagnosis; microbiology
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2007;42(11):852-855
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a reliable, rapid assay for detecting pathogenic aspergillus species in fungal sinusitis.
METHODSThirty-seven formalin-fixed and paraffin-embedded surgical tissue specimens from patients with fungal sinusitis were used in the present study. The aspergillus specific oligonucleotide probe was designed, commercially synthesized, and digoxigenin-labeled. Twenty-three-base oligonucleotides was selected that was complementary to 18S ribosomal RNA sequences (18S-1 probe) for detecting medically important aspergillus species.
RESULTSIn situ hybridization for aspergillus rRNA was positive in 28 cases with the 18S-1 probe. Compared with HE (21) and methenamine-silver stain (23).
CONCLUSIONIn situ hybridization provides rapid and accurate identification for fungal organism in tissues, and may be useful if cultures are negative or have not performed.
