Effects of hypoxia inducible factors-1alpha on autophagy and invasion of trophoblasts.
10.5653/cerm.2012.39.2.73
- Author:
Jong Ho CHOI
1
;
Hyun Jung LEE
;
Tae Hyun YANG
;
Gi Jin KIM
Author Information
1. Department of Biomedical Science, CHA University, Seoul, Korea. gjkim@cha.ac.kr
- Publication Type:Original Article
- Keywords:
Placenta;
Trophoblasts;
Hypoxia;
Invasiveness;
Programmed cell death type II
- MeSH:
Anoxia;
Apoptosis;
Autophagy;
Blotting, Western;
Cell Death;
Fluorescent Antibody Technique;
Hand;
Placenta;
Pregnancy;
RNA, Small Interfering;
Trophoblasts
- From:Clinical and Experimental Reproductive Medicine
2012;39(2):73-80
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: This study was undertaken to determine the effect of hypoxia inducible factor (HIF)-1alpha on the cell death, autophagy, and invasion of trophoblasts. METHODS: To understand the effect of HIF-1alpha, we inhibited HIF-1alpha using siRNA under normoxia and hypoxia conditions. Invasion assay and zymography were performed to determine changes in the invasion ability of HIF-1alpha. Western blotting and immunofluorescence were performed to determine some of the signal events involved in apoptosis and autophagy. RESULTS: There was no difference in cell death through the inhibition of HIF-1alpha expression by siRNA; however, the expression of LC3 and autophagosome formation increased. On the other hand, autophagy was increased, and the invasive ability of trophoblast cells decreased according to the inhibition of HIF-1alpha expression by siRNA. These experimental results mean that HIF-1alpha genes regulate the invasive ability of trophoblasts by increasing autophagy. CONCLUSION: This study contributes important data for understanding the mechanism of early pregnancy implantation and the invasive ability of trophoblasts by defining the relationship between the roles of HIF-1alpha and autophagy.
