Gene rearrangement analysis of papillary thyroid carcinoma.
- Author:
Yu-long WANG
1
;
Jiu-cun WANG
;
Dnan-shu LI
;
Yong-xue ZHU
;
Yi WU
;
Qing-hai JI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Carcinoma; Carcinoma, Papillary; Child; Female; Gene Rearrangement; Humans; Male; Middle Aged; Oncogene Proteins, Fusion; genetics; PTEN Phosphohydrolase; genetics; Protein-Tyrosine Kinases; genetics; Thyroid Neoplasms; genetics; pathology; Young Adult
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2007;42(12):929-933
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the characteristics of RET/PTC and H47PTEN rearrangement and the association between gene rearrangement and clinicopathological properties of thyroid carcinoma.
METHODSRearrangement of RET/PTC-1, RET/PTC-2, RET/PTC-3, ELKS-RET and H4-PTEN (H4/PTEN and PTEN/H4) was analyzed in 139 thyroid tumor tissues by using RT-PCR and sequencing.
RESULTSTwelve RET/PTC-1, 6 RET/PTC-3, 6 H4/PTEN and 7 PTEN/H4 were detected in 126 papillary thyroid carcinomas. In 3 cases, both RET/PTC and H4-PTEN were identified simultaneously. However, repeated experiments did not give the same results of H4-PTEN rearrangement. The overall frequency of rearrangement was 21.4% (27/126). The patients with gene rearrangement were younger (P = 0.02) and had a higher frequency of lymph node involvement (P = 0.02). High frequency of lateral neck lymph node involvement was detected in RET/PTC positive PTC (P < 0.01). PTEN/H4 rearrangement could also be detected in medullary thyroid carcinoma (2/5).
CONCLUSIONSH4-PTEN rearrangement can occur simultaneously with RET/PTC rearrangement in PTC. High predisposition to gene rearrangement is a characteristic of PTC. The patients of PTC with gene rearrangement are younger and have a higher frequency of lymph node involvement.
