Expression of alpha-v integrin subunit in the laryngeal and hypopharyngeal squamous cell carcinomas and its relationship with tumor angiogenesis
10.3321/j.issn:1673-0860.2008.01.012
- VernacularTitle:整合素αv亚基在喉和下咽鳞状细胞癌中表达及其与肿瘤血管发生的相关性
- Author:
Jian-Guang LU
1
;
Yu-Li XIAO
;
Xuan KAN
;
De-Jun JIN
Author Information
1. 哈尔滨医科大学附属第二医院
- Keywords:
Laryngeal neoplasms;
Hypopharyngeal neoplasms;
Receptors,vitronectin
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2008;43(1):46-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of integrin alpha-v subunit in laryngeal and hypopharyngeal squamous cell carcinomas(LHSCC) and to correlate the expression ratio with clinic and pathologic features of LHSCC,meanwhile,to investigate the relationship between the expression of integrin alpha-v subunit and tumor angiogenesis.Methods A tissue microarray of LHSCC was designed and made.Using this microarray,the expression of integrin alpha-v subunit in LHSCC was studied by immunohistochemistry,and the expression disparity in different clinic and pathologic staging of LHSCC was analyzed. The immunohistochemical staining of CD105 was done in the same microarray, the intratumor microvessel density(IMVD) was calculated by CD105 staining. The relationship between integrin alpha-v subunit expression and the IMVD was analyzed. Results In primary cancer tissue,the expression ratio of integrin alpha-v subunit was 68.0%(51/75), significantly higher than normal tissue beside cancer (10.3%,3/29,x2=28.68,P＜0.001);the expression ratio of integrin alpha-v subunit in lymph node metastatic carcinoma was 100.00%(20/20),significantly higher than normal tissue(x2=38.77,P＜0.001)and primary cancer tissue(x2=12.69,P＜0.05);in group with lymph node metastasis,the expression ratio of integrin alpha-v subunit was significantly higher than group without lymph node metastasis (x2=10.87,P＜0.001);the IMVD of the group with integrin alpha-v subunit positive expression was significantly higher than the group with integrin alpha-v subunit negative expression(P＜0.001).Conclusions There was significant relationship between integrin alpha-v subunit expression and lymphatic metastasis of LHSCC. Overexpression of the integrin alpha-v subunit may have contributed to the tumor comangiogenesis and lead to lymphatic metastasis.Integrin alpha-v subunit may become a novel lymphatic metastasis marker of LHSCC.
