Concurrent Assay for Four Bacterial Species Including Alloiococcus Otitidis in Middle Ear, Nasopharynx and Tonsils of Children with Otitis Media with Effusion: A Preliminary Report.
- Author:
Emine AYDIN
1
;
Eren TASTAN
;
Mihriban YUCEL
;
Filiz AYDOGAN
;
Esra KARAKOC
;
Necmi ARSLAN
;
Yunus KANTEKIN
;
Munir DEMIRCI
Author Information
- Publication Type:Original Article
- Keywords: Alloiococcus otitidis; Otitis media with effusion; Polymerase chain reaction; Culture; Nasopharynx; Tonsil
- MeSH: Bacteria; Child; Colon; Ear, Middle; Haemophilus influenzae; Humans; Influenza, Human; Moraxella (Branhamella) catarrhalis; Multiplex Polymerase Chain Reaction; Nasopharynx; Otitis; Otitis Media; Otitis Media with Effusion; Palatine Tonsil; Pneumonia; Polymerase Chain Reaction; Prevalence; Streptococcus pneumoniae
- From:Clinical and Experimental Otorhinolaryngology 2012;5(2):81-85
- CountryRepublic of Korea
- Language:English
- Abstract: OBJECTIVES: To detect the prevalences of Alloiococcus otitidis, as well as Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis in children with chronic otitis media with effusion (OME) and to simultaneously investigate the colonization of these bacteria in the nasopharynx and palatine tonsils of these patients. METHODS: The study included 34 pediatric patients with OME, and 15 controls without OME. In the study group, A. otitidis, H. influenzae, S. pneumoniae, and M. catarrhalis were investigated in the samples obtained from middle ear effusions (MEE), nasopharyngeal swabs (NPS) and tonsillar swabs (TS), using multiplex polymerase chain reaction (PCR) and conventional culture methods. Only the samples obtained from NPS and TS were studied with the same techniques in the control group. RESULTS: A. otitidis was isolated only in MEE and only with multiplex PCR method. A. otitidis, S. pneumoniae, M. catarrhalis, H. influenzae were identified in 35%, 8.8%, 8.8%, and 2.9%, respectively, in 34 MEE. A. otitidis was not isolated in NPS or TS of the study and the control groups. CONCLUSION: The prevalence of A.otitidis is high in children with OME and A.otitidis doesn't colonize in the nasopharynx or tonsil.
