Protective effect of maternal immunoglobulin G against NMDA-induced neurotoxicity on hippocampus neurons.
- Author:
Hong-mei WANG
1
;
Li-ping ZOU
;
Wei-hua ZHANG
;
Min ZENG
;
Chun-li ZHAO
;
Jun-yan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Cell Death; drug effects; Cell Survival; drug effects; Cells, Cultured; Excitatory Amino Acid Agonists; Female; Hippocampus; cytology; drug effects; metabolism; pathology; Immunity, Maternally-Acquired; immunology; Immunoglobulin G; biosynthesis; isolation & purification; pharmacology; Immunologic Factors; biosynthesis; isolation & purification; pharmacology; L-Lactate Dehydrogenase; analysis; biosynthesis; Male; N-Methylaspartate; Neurons; drug effects; metabolism; pathology; Organ Culture Techniques; Pregnancy; Rats; Rats, Wistar
- From: Chinese Journal of Pediatrics 2006;44(5):374-376
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate possible protective effect of maternal immunoglobulin G (IgG) against N-methyl-D-aspartate-mediated neurotoxicity on primary-cultured rat hippocampal neurons and the mechanism of the effect.
METHODSAn in vitro system had been developed for the study of hippocampal neurons. Intracellular lactic dehydrogenase (LDH) release was used as a marker to measure the rates of neuronal damage. The cells were stained with Trypan blue to measure the rate of neuronal death.
RESULTSN-methyl-D-aspartate (NMDA) at a concentration of 50 micromol/L resulted in increased release of LDH and the cell mortality (P < 0.01, respectively). Maternal IgG of different concentration (10 mg/L, 100 mg/L) inhibited NMDA-induced intracellular LDH release (P < 0.01, respectively) and cell mortality (P < 0.05, 0.01, respectively), and larger dose had stronger effect (P < 0.05).
CONCLUSIONSMaternal IgG had protective effect on primary-cultured rat hippocampal neurons injured by NMDA and the effect was dose-dependent.
