Effects of RhoA gene silencing by RNA interference on invasion of tongue carcinoma.
- Author:
Zhenggang CHEN
;
Yong-ping TANG
;
Lei TONG
;
Ying WANG
;
Yuan ZHOU
;
Qimin WANG
;
Jinhong HAN
;
Zongxuan HE
;
Yixiang LIAO
;
Bing FAN
;
Rong-hai ZOU
;
Jian ZHANG
;
Xiaofeng SUN
;
Guoxin YAN
- Publication Type:Journal Article
- MeSH: Carcinoma, Squamous Cell; genetics; metabolism; pathology; Cell Line, Tumor; Down-Regulation; Galectin 3; metabolism; Gene Silencing; Humans; Matrix Metalloproteinase 9; metabolism; RNA Interference; RNA, Messenger; metabolism; RNA, Small Interfering; genetics; Tongue Neoplasms; genetics; metabolism; pathology; Transfection
- From: West China Journal of Stomatology 2016;34(2):183-188
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of RhoA down-regulation by RNA interference on the invasion of tongue carcinoma Tca8113 and SCC-4.
METHODSDetermination of the human RhoA sequence as well as the design and constructionof a short specific small interfering RNAs (siRNA) were performed. The siRNA of RhoA gene was transfected into humantongue squamous cell carcinoma Tca8113 and SCC-4 cells line by Lipofectamine 2000. Quantitative real-time polymerasechain reaction was used to examine the mRNA expressionlevels of RhoA. Protein expressions of mRNA, galectin-3,and matrix metalloproteinase (MMP)-9 were evaluated byWestern blot. Transwell invasion assay was performed toassess the invasion ability of tongue carcinoma.
RESULTSRhoA expressions in Tca8113 and SCC-4 cells were reducedsignificantly after transfection of RhoA-siRNA. Protein levels f galectin-3 and MVP-9 were also down-regulated significantly. Invasion ability was inhibited as well.
CONCLUSIONRhoA-siRNA can effectively inhibit RhoA expression in Tca8113 and SCC-4 cells. The invasion ability of tongue carcinoma cells decreased with down-regulation of the protein expressions of galectin-3 and MMP-9, indicating that RhoA-siRNA can inhibit invasion of tongue carcinoma. Results show that RhoA may play an important role in the processes of invasion and metastasis of tongue carcinoma.