Treated dentin matrix enhances proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells.
- Author:
Hefeng YANG
;
Yu HU
;
Jingjing SUN
;
Weihua GUO
;
Weidong TIAN
;
Song LI
- Publication Type:Journal Article
- MeSH: Bone Marrow Cells; physiology; Bone and Bones; Calcium Phosphates; Cell Differentiation; Collagen Type I; Core Binding Factor Alpha 1 Subunit; Dentin; physiology; Hematopoietic Stem Cells; Humans; Mesenchymal Stromal Cells; physiology; Osteogenesis; Tissue Engineering
- From: West China Journal of Stomatology 2016;34(3):281-285
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe effect of treated dentin matrix (TDM) to the proliferation and osteogenesis differentiation of bone marrow mesenchymal stem cells (BMSCs) is evaluated in vitro.
METHODSTDM leaching solution was prepared by dentine particles suffering from gradient demineralization. Human BMSCs were isolated and cultivated, and subsequently cultivated in the TDM leaching solution. The proliferation of BMSCs was detected by CCK-8. The osteogenesis-related proteins, including collagen type I (Col I) and runt-related transcription factor-2 (Runx2), were extracted and detected by Western blot after a 7-day culture.
RESULTSCompared with the control group and hydroxyapatite (HA)/β-tricalcium phosphate (βTCP) group, the proliferation of BMSCs cultivated in TDM leaching solution was significantly improved. The expression of Col I and Runx2 obviously increased after the 7-day cultivation in TDM leaching solution.
CONCLUSIONTDM can promote the proliferation and osteogenesis differentiation of BMSCs, implying the feasibility of the application in bone tissue engineering.