Periodontal inflammation affects the mechanical and immune barrier functions of mice gut.
- Author:
Meilian XIE
1
;
Ting YU
2
Author Information
- Publication Type:Journal Article
- Keywords: gut microbiota; inflammatory bowel diseases; mechanical barrier; periodontitis
- MeSH: Alveolar Bone Loss; Animals; Inflammation; Male; Mice; Mice, Inbred C57BL; Periodontitis; Phylogeny; Porphyromonas gingivalis; RNA, Ribosomal, 16S
- From: West China Journal of Stomatology 2016;34(4):414-418
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of periodontal inflammation on the functions of gut barrier (ecological barrier, mechanical barrier, and immune barrier) in mice.
METHODSTwenty male C57BL/6J mice were randomly divided into perio-dontitis (P) or control (C) groups. The P group was subjected under a 10-day ligation with Porphyromonas gingivalis to induce periodontitis, whereas the C group was ligated with sham. Maxillae were obtained to assess alveolar bone loss. The phylogenetic structure and diversity of microbial communities in the gut were analyzed by 16s rRNA pyrosequencing. Immunohisto-chemical analysis was performed to determine the expressions of occludin, claudin2, and NOD2 in the ileum.
RESULTSCom-pared with the C group, the P group displayed significant alveolar bone loss (P<0.001). In addition, no significant influence on the main phyla and genus Parabacteroides of the two groups was observed (P>0.05). However, the ileum of the P group showed significantly upregulated occludin, claudin2, and NOD2 (P=0.039, P=0.011, and P=0.039, respectively).
CONCLUSIONSPeriodontal inflammation influences to some extent the mechanical and immune barrier functions of the mice gut. .
