Influences of vibration on rapid osteogenic response of osteoblasts.
- Author:
Zhuoli ZHU
1
;
Ling ZHANG
2
Author Information
- Publication Type:Journal Article
- Keywords: mitochondrial morphology; osteogenic differentiation; reactive oxygen species; vibration
- MeSH: Alkaline Phosphatase; Animals; Cell Line; Core Binding Factor Alpha 1 Subunit; Down-Regulation; Mice; Osteoblasts; Osteogenesis; Vibration
- From: West China Journal of Stomatology 2017;35(1):68-72
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis study investigated the rapid response of osteoblasts, which were derived from low-magnitude high-frequency vibration (LMHFV). Refractory period-derived memory response was also observed.
METHODSMC3T3-E1 cells were incubated and received LMHFV stimulation (0.49 g, 40 Hz) for 30 min. After application of LMHFV, mRNA levels of earlier osteogenic differentiation markers Runt-related transcription factor 2 (Runx2), collagen typeⅠ(Col-Ⅰ), and alkaline phosphatase (ALP) were immediately detected by real-time fluorescence quantitative polymerase chain reaction in the absence or presence of antioxidant. Simultaneously, concentrations of mitochondrial reactive oxygen species (ROS) and average mitochondrial length were also measured.
RESULTSOsteoblasts in the vibration group showed decreased gene expressions of Runx2, Col-Ⅰ, and ALP (P<0.01) and increased levels of mitochondrial ROS (P<0.01) and shortened mitochondria (P<0.01), whereas antioxidant treatment resulted in recovery from changes in the above indicators (P<0.01).
CONCLUSIONSLMHFV can downregulate mRNA levels of early osteogenic differentiation markers, promote ROS generation, and mitochondrial fission. .
