Assignment of CCR7 gene to chicken chromosome 27 by radiation hybrid panel mapping.
- Author:
Yong TIAN
1
,
2
;
Li-zhi LU
;
Yan FU
;
Zheng-rong TAO
;
Jun-da SHEN
;
De-qian WANG
;
Ai-ping YUAN
;
Zhao-zheng YIN
Author Information
1. Laboratory of Animal Genetics, College of Animal Sciences, Zhejiang University, Hangzhou 310029, China
2. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Base Sequence;
Chickens;
genetics;
Chromosomes;
genetics;
Humans;
Molecular Sequence Data;
Radiation Hybrid Mapping;
methods;
Receptors, CCR7;
Receptors, Chemokine;
genetics;
Sequence Analysis, DNA;
methods;
Sequence Homology, Nucleic Acid
- From:
Journal of Zhejiang University. Science. B
2007;8(5):314-317
- CountryChina
- Language:English
-
Abstract:
The protein encoded by CC chemokine receptor 7 (CCR7) is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. To map the CCR7 gene in chicken chromosome, a 6 000 rads chicken-hamster radiation hybrid panel (ChickRH6) was used. PCR of samples from ChickRH6 revealed that the location of CCR7 gene is linked to the maker SEQ0347 (6 cR away) with LOD score of 16.6 and that the marker SEQ0347 is located on chromosome 27 at 27 cR of RH (radiation hydrid) map. We compared the corresponding human mRNA sequence with the predicted coding sequence of chicken CCR7 gene, and found that the assembled contig shared a high percentage of similarity with that of the human gene.
